《植物生理学报》 2011, 47(1): 57-62

拟南芥 AZI1 基因对酿酒酵母细胞生长和蒜薹灰霉菌侵染的抑制作用

张欣^1, 徐之艳¹, Michael Schläppi², 徐子勤^1,*

1 西北大学生命科学学院, 西部资源与现代生物技术省部共建教育部重点实验室, 陕西省生物技术重点实验室, 西安 710069;

通信作者：徐子勤；E-mail: ziqinxu@nwu.edu.cn；Tel: 029-88303484

摘　要：

本工作采用酿酒酵母细胞表达载体pESC 和植物细胞表达载体pPZP211 分析了拟南芥AZI1 基因对真菌的抗性功能。 半乳糖诱导产生的 AZI1 蛋白可以使酵母细胞的生长能力明显降低。DAB 和台酚蓝染色结果显示用蒜薹灰霉菌孢子处理 Col-0 野生型植株叶片后被侵染部位只能产生少量H2O2, 病原体可以扩散, 而AZI1基因过表达植株叶片在侵染部位有大量 H2O2 产生, 着色较深, 表明转化体能够以局部细胞的死亡来阻止病原体侵染周围的细胞。在 Col-0 野生型植株中, AZI1 基 因的表达受外源水杨酸诱导, 24 h 后达到峰值。以上结果说明 AZI1 基因在拟南芥对生物胁迫因素的应答过程中具有重要 作用。

关键词：AZI1基因; 过表达; 酿酒酵母; 蒜薹灰霉菌; 水杨酸; H2O2

收稿：2010-08-16   修定：2010-10-22

资助：国家自然科学基金(30870194)、陕西省重点实验室研究计划(08JZ70; 2010JS090)和陕西省科学技术研究发展计划(攻关计划2010K16-04-01)。

Inhibition of Arabidopsis AZI1 to the Growth of Saccharomyces cerevisiae and Infection of Botrytis cinerea Pers. ex of Garlic Sprout

ZHANG Xin¹, XU Zhi-Yan¹, Michael Schläppi², XU Zi-Qin^1,*

¹Provincial Key Laboratory of Biotechnology of Shaanxi, Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, China; ²Department of Biological Sciences,Marquette University, Milwaukee, WI 53233, USA

Corresponding author: XU Zi-Qin; E-mail: ziqinxu@nwu.edu.cn; Tel: 029-88303484

Abstract:

Resistance of Arabidopsis AZI1 to fungus was analyzed with Saccharomyces cerevisiae expression vector pESC and plant expression vector pPZP211. Synthesis of AZI1 after induction with galactose resulted in remarkable decrease of the growth ability of yeast cells. DAB and trypan blue staining showed that only small amounts of H₂O₂ were produced at the infection sites of Botrytis cinerea spores and the pathogens could spread to surrounding cells in leaves of wild-type Col-0 plants. In contrast to this, the infection sites on leaves of AZI1 overexpression Col-0 plants accumulated large amounts of H₂O₂ and were stained intensively by DAB, indicating the transformants could prevent the spread of pathogen by the death of local cells around the infection sites. In wild-type Col-0 plants, the expression of AZI1 could be induced by salicylic acid and peaked after 24 h. All these results suggested that AZI1 play an important role in defense system of Arabidopsis against biotic stresses.

Key words: AZI1; overexpression; Saccharomyces cerevisiae; Botrytis cinerea Pers. ex of garlic sprout; salicylic acid; H₂O₂